生命科学部Faculty of Bioscience and Applied Chemistry
BLS300YB（生物科学 / Biological science 300）蛋白工学Protein Engineering
常重 アントニオAntonio TSUNESHIGE
授業コードなどClass code etc
| 学部・研究科Faculty/Graduate school | 生命科学部Faculty of Bioscience and Applied Chemistry |
| 添付ファイル名Attached documents | |
| 年度Year | 2022 |
| 授業コードClass code | H7040 |
| 旧授業コードPrevious Class code | |
| 旧科目名Previous Class title | |
| 開講時期Term | 秋学期授業/Fall |
| 曜日・時限Day/Period | 木4/Thu.4 |
| 科目種別Class Type | |
| キャンパスCampus | 小金井 |
| 教室名称Classroom name | 各学部・研究科等の時間割等で確認 |
| 配当年次Grade | |
| 単位数Credit(s) | |
| 備考（履修条件等）Notes |
成績優秀者の他学部科目履修制度で履修する学 生：教員の受講許可が必要(オンライン授業の場合は、学習支援システムで許可を得るようにする) |
| 他学部公開科目Open Courses | |
| 他学部公開（履修条件等）Open Courses (Notes) | |
| グローバル・オープン科目Global Open Courses | |
| 成績優秀者の他学部科目履修制度対象Interdepartmental class taking system for Academic Achievers | ○ |
| 成績優秀者の他学部科目履修（履修条件等）Interdepartmental class taking system for Academic Achievers (Notes) | |
| 実務経験のある教員による授業科目Class taught by instructors with practical experience | ○ |
| SDGsCPSDGs CP | |
| アーバンデザインCPUrban Design CP | |
| ダイバーシティCPDiversity CP | |
| 未来教室CPLearning for the Future CP | |
| カーボンニュートラルCPCarbon Neutral CP | |
| 千代田コンソ単位互換提供（他大学向け）Chiyoda Campus Consortium | |
| カテゴリー＜生命科学部＞Category | 学部共通科目 |
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授業の概要と目的（何を学ぶか）Outline and objectives
This course is designed to give students a succinct, yet solid knowledge of protein structure, and the many techniques to alter and produce them, with special emphasis on the design of structures with . Starting from a presentation of their basic physicochemical properties. The course will emphasize on the various techniques applied, ranging from chemical modification in already known proteins to the design and creation of new protein motifs.
到達目標Goal
The enrolled student will learn first the basic physico-chemical properties and functions of proteins, including those of amino acids and peptides. Later, the student will learn the different goals of protein engineering, more specifically, protein designand its basic techniques and applications.
この授業を履修することで学部等のディプロマポリシーに示されたどの能力を習得することができるか（該当授業科目と学位授与方針に明示された学習成果との関連）Which item of the diploma policy will be obtained by taking this class?
DP2
授業で使用する言語Default language used in class
英語 / English
授業の進め方と方法Method(s)（学期の途中で変更になる場合には、別途提示します。 /If the Method(s) is changed, we will announce the details of any changes. ）
As conditions permit, classes will be conducted in the form of presential lectures. Handouts will be available through the Hoppii system. Therefore, in most cases, bringing a personal computer to classes is greatly advised.
As assessment of learning, quizzes and homework will be assigned periodically, and their solutions with commentaries will be explained in following sessions.
Submission of reports will be requested and use as feedback.
Active participation of students is encouraged.
アクティブラーニング（グループディスカッション、ディベート等）の実施Active learning in class (Group discussion, Debate.etc.)
あり / Yes
フィールドワーク（学外での実習等）の実施Fieldwork in class
なし / No
授業計画Schedule
授業形態/methods of teaching：対面/face to face
※各回の授業形態は予定です。教員の指示に従ってください。
1[対面/face to face]：Introduction.
Proteins. Scope of this course.
2[対面/face to face]：Structure of proteins (I).
From amino acids, to peptides, proteins, and protein macro-complexes.
Basic concepts in protein structure.
2[対面/face to face]：Structure of proteins (II).
Physicochemical properties of proteins. Stabilization forces of protein structures.
The hydrophobic collapse.
4[対面/face to face]：Structural analysis of proteins. Chemical modifications of proteins.
Learning from nature. Use of databases. Visualization of protein structures. Use of chemical labels.
5[対面/face to face]：Protein purification
Qualitative and quantitative techniques employed in protein purification and characterization
6[対面/face to face]：The core of this course: Protein Design
How to design and create proteins from scratch. Taming the stabilizing forces in protein constructs.
Analyzing examples from nature.
7[対面/face to face]：De novo Design of Proteins (I).
The alpha helix. How to use the helical wheel.
Alternative devices.
8[対面/face to face]：De novo Design of Proteins (II).
Design of tertiary structures in proteins.
9[対面/face to face]：De novo Design of Proteins (III).
The Merrifield method of protein synthesis.
Protein production without an organism.
10[対面/face to face]：Protein Denaturation.
Thermodynamic aspects of protein denaturation.
11[対面/face to face]：Protein Refolding.
The still unsolved problem of protein refolding.
12[対面/face to face]：Monoclonal Antibodies.
Basic immunology. How this technique lead to a Nobel Prize.
13[対面/face to face]：Proteins in Bio-Medicine.
Introduction of engineered proteins with applications in Medicine.
14[対面/face to face]：The Future of Protein Engineering
Beyond the helix bundle motif.
授業時間外の学習（準備学習・復習・宿題等）Work to be done outside of class (preparation, etc.)
【本授業の準備・復習等の授業時間外学習は、4時間を標準とする】
Prior classes, handouts and references will be distributed through the system Hoppii. The enrolled student is encouraged to read the provided material before classes.
Quizzes and problems to be solved after classes will be distributed periodically, and their responses scored accordingly. Solution to these will be given in following classes.
テキスト（教科書）Textbooks
The textbook shown below can be used as a textbook, although this does not cover all the topics presented in class.
改訂「酵素－科学と工学」虎屋哲夫等，講談社 （2012）
参考書References
Handouts and references will be available in digital form from the system Hoppii.
成績評価の方法と基準Grading criteria
Final exam (or equivalent): 50%; assignments and reports: 25%; active participation in class: 25%.
学生の意見等からの気づきChanges following student comments
The syllabus for the current year has been updated to focus on selected points that required more emphasis.
学生が準備すべき機器他Equipment student needs to prepare
Personal computer to access the Hoppii systems. All references will be made available in digital format.