Faculty of Bioscience and Applied Chemistry

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BLS300YB(生物科学 / Biological science 300)
Protein Engineering


Class code etc
Faculty/Graduate school Faculty of Bioscience and Applied Chemistry
Attached documents
Year 2022
Class code H7040
Previous Class code
Previous Class title
Term 秋学期授業/Fall
Day/Period 木4/Thu.4
Class Type
Campus 小金井
Classroom name 各学部・研究科等の時間割等で確認
Notes 成績優秀者の他学部科目履修制度で履修する学
Open Program
Open Program (Notes)
Global Open Program
Interdepartmental class taking system for Academic Achievers
Interdepartmental class taking system for Academic Achievers (Notes)
Class taught by instructors with practical experience
Urban Design CP
Diversity CP
Learning for the Future CP
Carbon Neutral CP
Chiyoda Campus Consortium
Category 学部共通科目

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Outline and objectives

This course is designed to give students a succinct, yet solid knowledge of protein structure, and the many techniques to alter and produce them, with special emphasis on the design of structures with . Starting from a presentation of their basic physicochemical properties. The course will emphasize on the various techniques applied, ranging from chemical modification in already known proteins to the design and creation of new protein motifs.


The enrolled student will learn first the basic physico-chemical properties and functions of proteins, including those of amino acids and peptides. Later, the student will learn the different goals of protein engineering, more specifically, protein designand its basic techniques and applications.

Which item of the diploma policy will be obtained by taking this class?


Default language used in class

英語 / English

Method(s)(学期の途中で変更になる場合には、別途提示します。 /If the Method(s) is changed, we will announce the details of any changes. )

As conditions permit, classes will be conducted in the form of presential lectures. Handouts will be available through the Hoppii system. Therefore, in most cases, bringing a personal computer to classes is greatly advised.
As assessment of learning, quizzes and homework will be assigned periodically, and their solutions with commentaries will be explained in following sessions.
Submission of reports will be requested and use as feedback.
Active participation of students is encouraged.

Active learning in class (Group discussion, Debate.etc.)

あり / Yes

Fieldwork in class

なし / No


授業形態/methods of teaching:対面/face to face


1[対面/face to face]:Introduction.

Proteins. Scope of this course.

2[対面/face to face]:Structure of proteins (I).

From amino acids, to peptides, proteins, and protein macro-complexes.
Basic concepts in protein structure.

2[対面/face to face]:Structure of proteins (II).

Physicochemical properties of proteins. Stabilization forces of protein structures.
The hydrophobic collapse.

4[対面/face to face]:Structural analysis of proteins. Chemical modifications of proteins.

Learning from nature. Use of databases. Visualization of protein structures. Use of chemical labels.

5[対面/face to face]:Protein purification

Qualitative and quantitative techniques employed in protein purification and characterization

6[対面/face to face]:The core of this course: Protein Design

How to design and create proteins from scratch. Taming the stabilizing forces in protein constructs.
Analyzing examples from nature.

7[対面/face to face]:De novo Design of Proteins (I).

The alpha helix. How to use the helical wheel.
Alternative devices.

8[対面/face to face]:De novo Design of Proteins (II).

Design of tertiary structures in proteins.

9[対面/face to face]:De novo Design of Proteins (III).

The Merrifield method of protein synthesis.
Protein production without an organism.

10[対面/face to face]:Protein Denaturation.

Thermodynamic aspects of protein denaturation.

11[対面/face to face]:Protein Refolding.

The still unsolved problem of protein refolding.

12[対面/face to face]:Monoclonal Antibodies.

Basic immunology. How this technique lead to a Nobel Prize.

13[対面/face to face]:Proteins in Bio-Medicine.

Introduction of engineered proteins with applications in Medicine.

14[対面/face to face]:The Future of Protein Engineering

Beyond the helix bundle motif.

Work to be done outside of class (preparation, etc.)

Prior classes, handouts and references will be distributed through the system Hoppii. The enrolled student is encouraged to read the provided material before classes.
Quizzes and problems to be solved after classes will be distributed periodically, and their responses scored accordingly. Solution to these will be given in following classes.


The textbook shown below can be used as a textbook, although this does not cover all the topics presented in class.

改訂「酵素-科学と工学」虎屋哲夫等,講談社 (2012)


Handouts and references will be available in digital form from the system Hoppii.

Grading criteria

Final exam (or equivalent): 50%; assignments and reports: 25%; active participation in class: 25%.

Changes following student comments

The syllabus for the current year has been updated to focus on selected points that required more emphasis.

Equipment student needs to prepare

Personal computer to access the Hoppii systems. All references will be made available in digital format.